In order to investigate experimentally that the effect of aqua-acupuncture solution of Paeonia lactiflora on arthritis of mice induced by collagen II, the author performed several experimental items : that is increase, paw thickness, DTH, weight of spleen, hematological change, expression of CD4^+, CD8^+, CD19^+, gene expression of IL-1, IL-6, IL-12, IFN= γ, TNF, proliferation of synovial cells and cytotoxicity.
The results were obtained as follows;
1. Inhibitory effects of aqua-acupuncture solution of Paeonia lactiflora on arthritis induced by collgen II .
1) In incidence, paw edema, AI and DTH were inhibited as compared with control group.
2) The splenic weight was increased and the number of leukocytes was decreased as compared with control group.
3) The number of CD4^+, CD8^+ activated cells and surface-receptor expression were increased as compared with control group.
4) In hematological change, total protein, creatinine and LDH were decreased significantiy as compared with control group.
2. FACS analysis on normal BABL/c of spenic cells treated with aqua-acupuncture solution of Paeonia lactiflora.
1) Aqua-acupuncture solution of Paeonia lactiflora activated adhesive splenic cells of mice morphologically in vitro.
2) Aqua-acupuncture solution of Paeonia dactiflora enhanced the gene expression of IL-12 and also enhanced that of interferon- γ remarkably.
3) Aqua-acupuncture solution of Paeonia lactiflora reduced the number of CD4^+, CD8^+, CD19^+ activated cells and their surface-receptor expression as compared with control group.
3. Effects of aqua-acupuncture solution of Paeonia lactiflora on human synovial cells.
1) In cytotoxicity against synovial cells, aqua-acupuncture solution of Paeonia lactiflora didn´t show cytotoxicity at concentration of 10-100㎍/㎖ but showed significantly at concentration of 200-400㎍/㎖ as compared with control group.
2) Aqua-acupuncture solution of Paeonia lactiflora reduced the gene expression of IL-6, IL-1β and TNF- α.
3) Aqua-acupuncture solution of Paeonia lactiflora inhibited proliferation of synovial cells at concentration of 100 and 200㎍/㎖.
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